Technology
Biodegradable HPAE polymer technology powering transfection reagents.
BrPERfect® and TransPi™ products are based on hyperbranched poly(β-amino ester)s — biodegradable cationic polymers engineered for nucleic acid complexation and cellular delivery.
Mechanism
From polymer to cargo release.
- 01
Nucleic acid complexation
HPAE polymers bind and condense DNA / RNA cargo into stable polyplexes.
- 02
Nanoparticle formation
Hyperbranched architecture drives consistent, sub-micron particle assembly.
- 03
Cellular uptake
Positively charged polyplexes interact with cell membranes for efficient internalisation.
- 04
Endosomal escape
Proton-sponge behaviour enables cargo release from endocytic compartments.
- 05
Cargo release
Intracellular release supports gene expression, editing or protein delivery.
- 06
Hydrolytic degradation
Biodegradable backbone reduces long-term material burden vs. non-degradable polymers.
Platform features
What sets the HPAE platform apart.
Hyperbranched architecture
Greater functional-group density and a broader design space than linear polymer systems.
Biodegradable polymer design
Hydrolysable backbone reduces long-term material burden.
Efficient DNA complexation
Strong binding and condensation of nucleic acids into stable polyplexes.
Scalable synthesis
Controllable, scalable synthesis supports batch consistency and process development.
Formulation optimisation
Structure, DNA concentration, ratio, buffer and mixing can be tuned per application.
Wide application potential
DNA, RNA, RNP, protein delivery research, viral vector production and future gene delivery concepts.
Platform ownership
PolyGene Bio's products are built on Branca Bunús' proprietary HPAE gene delivery platform.
For deeper platform science, IP, publications, patents and therapeutic development, visit Branca Bunús. PolyGene Bio focuses on the commercial product side — specifications, performance data, protocols and technical support for BrPERfect® and TransPi™ reagents.