PolyGene Bio commercializes BrPERfect® and TransPi™ transfection reagent products for researchers, biomanufacturers and partners worldwide — built on Branca Bunús' proprietary biodegradable HPAE gene delivery technology.
PolyGene Bio × Branca Bunús
Commercialization + Innovation · Advancing Gene Delivery, Together.
HPAE platform · owned by Branca Bunús
Hyperbranched poly(β-amino ester) polyplex → cellular uptake → endosomal escape → cargo release.
The gene delivery ecosystem
Branca Bunús owns and develops the proprietary HPAE gene delivery platform. PolyGene Bio commercializes the transfection reagent products built on it — with the specifications, protocols, performance data and support that scientists and manufacturers rely on.
Platform owner & technology innovator
The technology at the core
Biodegradable hyperbranched poly(β-amino ester) technology enabling efficient nucleic acid complexation, nanoparticle formation, cellular uptake, endosomal escape and intracellular release.
How the platform powers our productsCommercial product & support partner
PolyGene Bio website purpose
PolyGene Bio helps scientists and manufacturers find the best transfection reagents with reliable performance, easy workflows and trusted support.
Key message
High-performance, reliable and scalable transfection reagents for every step of bioproduction and research.
Product families
From GMP-grade viral vector production to specialised research transfection, every product is built on the same biodegradable HPAE chemistry.
BrPERfect®
High-efficiency DNA transfection reagent for transient viral vector production.
BrPERfect®
HPAE-based DNA transfection reagent for transient protein & antibody expression.
TransPi™
Research-use HPAE-based DNA transfection reagent for adherent cells.
TransPi™
Specialised research transfection reagent for brain-cell workflows.
Featured product series
High-performance transfection reagents for viral vector production, recombinant protein expression and industrial bioproduction workflows. Available in R&D and GMP grades.
Explore BrPERfect®Research-focused transfection reagents for adherent cells, primary cells, stem cells, brain cells, in vivo research delivery and specialised nucleic acid delivery workflows.
Explore TransPi™Applications
Adapted to your cell system, culture format and production goal — from lentivirus at scale to primary neurons.
LV · AAV5/AAV6 · retrovirus · pseudovirus
Designed for high-efficiency transient viral vector production with quick-mix preparation, stable complexes and scale-up friendly workflows in HEK293 suspension and adherent systems.
Recombinant proteins · mAbs · bispecifics
Transient expression workflows in 293 and CHO cells for biopharma, CROs and protein expression teams needing throughput and batch consistency.
Reporter assays · functional screening · method dev
Adherent-cell plasmid DNA transfection for research groups, with a simple quick-mix protocol and optimisable parameters per experiment.
Hard-to-transfect research models
Formulated HPAE reagents for delicate cell types where standard reagents struggle — from iPSCs to primary neurons.
Spleen · lung · fluorescent imaging
Research-use in vivo delivery reagents for preclinical gene delivery models, including fluorescent tracking for mouse in vivo imaging.
Non-viral gene delivery platform research
The HPAE platform supports DNA, RNA, siRNA, RNP, CRISPR/Cas9 and protein delivery research, enabling non-viral gene delivery investigations.
Designed for real workflows
Simple pipetting or brief vortexing — no incubation required for selected workflows.
Biodegradable HPAE chemistry designed to reduce long-term material burden.
Protocols for larger-volume complex preparation, batch consistency and documentation.
Animal-component-free formulations supporting GMP-grade options for BrPERfect®.
Technology
BrPERfect® and TransPi™ are built on hyperbranched poly(β-amino ester) chemistry — a biodegradable cationic polymer engineered for nucleic acid complexation, polyplex formation, cellular uptake, endosomal escape and intracellular cargo release.
Technology research
PolyGene Bio continues to advance polymer-based gene delivery research through controlled polymerisation, HPAE material development, formulation optimisation and transfection reagent application development.
Proprietary controlled polymerisation as the chemical foundation for HPAE material design.
HPAE polymers for DNA, RNA, RNP and protein delivery across research and production.
From polymer to product — formulation, QC and scale-up for BrPERfect® and TransPi™.
Cell-line, ratio, buffer and workflow optimisation with our scientific team.
Company events
Product launches, industry summits and user feedback from the PolyGene Bio / Branca Bunús ecosystem.
PolyGene Bio / Branca Bunús launched BrPERfect® for Virus, addressing critical pain points in transient viral vector production for lentivirus, AAV5, AAV6, retrovirus and pseudovirus workflows — adherent and suspension.
PolyGene Bio participated in the Future Cell Therapy Decision-Makers Summit (Sept 17–18, 2025), with industry discussions focused on in vivo CAR-T and emerging cell therapy technologies.
User feedback described strong GFP expression in 293T cells after transfection — visible green fluorescence observed in a T75 flask.
Featured references
Guanidyl-rich highly branched poly(β-amino ester)s for the delivery of dual CRISPR ribonucleoprotein for efficient large DNA fragment deletion.
Highly branched poly(β-amino ester) / CpG-depleted CFTR plasmid nanoparticles for non-viral gene therapy in lung cystic fibrosis disease.
News & updates
Launch update covering suspension and adherent HEK293 workflows, with source data reporting several-fold enhancement in lentiviral titers versus mainstream competitors.
Read moreConsolidated protocol and troubleshooting guide for LV, AAV5, AAV6, retrovirus and pseudovirus transient production.
Read moreParticipating in a cell therapy event focused on in vivo CAR-T and comparative analysis of cutting-edge technologies.
Read moreGet in touch
Tell us your cell line, culture format, target application and production goal. Our team will help you identify the most suitable product and optimisation route.