Protocols & Support
Product manuals, application protocols and optimisation guidance.
Transfection performance depends on cell line, culture format, DNA amount, reagent ratio, complex volume, buffer, mixing and harvest timing. Our team supports you in evaluating and optimising these parameters for your process.
Support areas
Where our team helps most.
- Suspension and adherent HEK293 workflows
- AAV and LV vector production
- Protein and antibody transient expression
- Adherent-cell DNA transfection
- siRNA transfection
- Primary / stem / brain cell transfection
- In vivo spleen / lung research delivery
- Reagent-to-DNA ratio optimisation
- Scale-up and complex preparation strategy
- Troubleshooting efficiency, viability and yield
Use Examples
Practical application examples and user feedback.
Explore practical application examples, user feedback and workflow scenarios for BrPERfect® and TransPi™ transfection reagents across research and bioproduction use cases.
TransPi™ · 293T
GFP expression visible to the naked eye
User feedback describes strong GFP expression in 293T cells after transfection — visible green fluorescence observed in a T75 flask.
BrPERfect® · Virus
Universal transient viral production
BrPERfect® for Virus supports LV, AAV5, AAV6, retrovirus and pseudovirus production across suspension and adherent HEK293 workflows.
BrPERfect® · Protein
High-throughput protein / mAb expression
BrPERfect® for Protein enables transient recombinant protein, mAb and bispecific antibody expression in 293 and CHO cells.
Documents
Product manuals & protocol library.
BrPERfect®
TransPi™ Mate
PolyGene Bio TransPi Series Product Manual
RequestTransPi Mate Adherent Cell Brief Instructions
RequestTransPi Mate Adherent Plasmid DNA Transfection Manual
RequestTransPi Mate Viral Transient Production Manual
RequestTransPi Mate Suspension-Protein Transient Production Manual
RequestTransPi Mate siRNA Transfection Manual
Request
FAQ
Technical questions, answered.
What is the core technology behind BrPERfect® and TransPi™ reagents?＋
The products are based on biodegradable hyperbranched poly(β-amino ester) polymers designed for efficient nucleic acid complexation and cellular delivery.
How long are the transfection complexes stable at room temperature?＋
Under recommended conditions, BrPERfect® for Virus complexes have been validated to remain stable for ≥3 h. BrPERfect® for Protein complexes are validated for ≥2 h. TransPi™ Mate complexes are designed to remain stable ≥3 h.
Can DPBS, PBS or serum-free medium be used instead of the supplied buffer?＋
For selected workflows and sensitive cells, protocol guidance allows testing DPBS, PBS or serum-free medium as alternatives to the provided mixing buffer.
Do bubbles affect transfection efficiency during mixing?＋
No — the protocols confirm bubbles do not affect efficiency. Rapid and thorough mixing is preferred over slow gentle inversion.
Are BrPERfect® products suitable for scale-up?＋
Yes. Workflows are designed for simple, rapid complex preparation, and protocols include guidance for larger-volume complex preparation and scale-up.
Are the products for human use?＋
TransPi™ Mate is for research use only and not for use in humans. Other products should be used according to product grade, documentation and customer qualification requirements.
What should I include when requesting technical support?＋
Cell line, culture format, DNA amount, reagent ratio, culture volume, medium system, target readout, production goal and any current performance issue.
Still troubleshooting?
Share your cell line, DNA amount, ratio, culture volume, medium and current issue — our scientific team will get back to you within 24 h.
Contact technical support